|
KMID : 0380019950100030323
|
|
Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 3 p.323 ~ p.333
|
|
|
Glucose Effects on Cell Growth, Antibody Production, and Cell Metabolism of Hybridoma Cells
|
|
Jeong Yeon-Ho
Shaw S. Wang
|
|
|
Abstract
|
|
|
|
The effects of glucose on cell growth kinetics, monoclonal antibody productivity, and cell metabolism of hybridoma cells were investigated. The mouse-mouse hybridoma cell line VIII H-8 producing mouse IgG;was used as a model system. Glucose showed substrate inhibition type dependence on specific growth rate. The maximum cell density increased as initial glucose concentration increased up to 4 g/ Q . Glucose showed a strong influence on cell death kinetics, and an inverse relationship between specific death rate and glucose concentration was found. Cell viability and monoclonal antibody production increased as initial glucose concentration increased. The specific glucose consumption rate increased with glucose concentration, and cumulative specific lactate production rate increased with increasing initial glucose concentration. The overall kinetics of ammonium ion production was almost invariant with respect to initial glucose concentration, while the cumulative specific ammonium ion production rate was dependent on initial glucose concentration.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|